Carbohydrate Composition Analysis in Xylem.
Identifieur interne : 001492 ( Main/Exploration ); précédent : 001491; suivant : 001493Carbohydrate Composition Analysis in Xylem.
Auteurs : Baocai Zhang [République populaire de Chine] ; Yihua Zhou [République populaire de Chine]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Cellulose, Glucides, Oses.
- composition chimique : Xylème.
- Chromatographie gazeuse-spectrométrie de masse.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Carbohydrates, Cellulose, Monosaccharides.
- chemistry : Xylem.
- Gas Chromatography-Mass Spectrometry.
Abstract
Gas chromatography-mass spectrometry (GC-MS) is a widely applied technique in carbohydrate composition analysis due to high accuracy and repeatability. As cell walls are the major carbohydrate pool in xylem tissues, the measurement of carbohydrate composition essentially constitutes an analysis of wall residues. Here, we describe the method for quantification of the contents of several common neutral monosaccharides and cellulose, the major components of cell walls in poplar xylem tissues, by GC-MS. The procedures for extraction of cell wall residues from xylem, generation of alditol derivatives, subsequent further separation on gas chromatograph, and detection by mass spectrometer are included.
DOI: 10.1007/978-1-4939-6722-3_15
PubMed: 28050838
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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As cell walls are the major carbohydrate pool in xylem tissues, the measurement of carbohydrate composition essentially constitutes an analysis of wall residues. Here, we describe the method for quantification of the contents of several common neutral monosaccharides and cellulose, the major components of cell walls in poplar xylem tissues, by GC-MS. The procedures for extraction of cell wall residues from xylem, generation of alditol derivatives, subsequent further separation on gas chromatograph, and detection by mass spectrometer are included.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28050838</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2018</Year><Month>04</Month><Day>26</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1544</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Carbohydrate Composition Analysis in Xylem.</ArticleTitle><Pagination><MedlinePgn>213-222</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-6722-3_15</ELocationID><Abstract><AbstractText>Gas chromatography-mass spectrometry (GC-MS) is a widely applied technique in carbohydrate composition analysis due to high accuracy and repeatability. As cell walls are the major carbohydrate pool in xylem tissues, the measurement of carbohydrate composition essentially constitutes an analysis of wall residues. Here, we describe the method for quantification of the contents of several common neutral monosaccharides and cellulose, the major components of cell walls in poplar xylem tissues, by GC-MS. The procedures for extraction of cell wall residues from xylem, generation of alditol derivatives, subsequent further separation on gas chromatograph, and detection by mass spectrometer are included.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Baocai</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yihua</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. yhzhou@genetics.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002241">Carbohydrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009005">Monosaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002241" MajorTopicYN="N">Carbohydrates</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008401" MajorTopicYN="N">Gas Chromatography-Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009005" MajorTopicYN="N">Monosaccharides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Alditol acetate</Keyword><Keyword MajorTopicYN="Y">Cellulose</Keyword><Keyword MajorTopicYN="Y">Gas chromatography</Keyword><Keyword MajorTopicYN="Y">Mass spectrometry</Keyword><Keyword MajorTopicYN="Y">Monosaccharide</Keyword><Keyword MajorTopicYN="Y">Neutral sugar</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28050838</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-6722-3_15</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Baocai" sort="Zhang, Baocai" uniqKey="Zhang B" first="Baocai" last="Zhang">Baocai Zhang</name></noRegion><name sortKey="Zhou, Yihua" sort="Zhou, Yihua" uniqKey="Zhou Y" first="Yihua" last="Zhou">Yihua Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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